Otimização das condições de extração de saponinas em Ampelozizyphus amazonicus usando planejamento experimental e metodologia de superfície de resposta

Autor: Fabiane M dos Santos, Jersika C Simões, Jefferson R. A Silva, Rosangela C Barthus, Ronei J Poppi, Ana C. F Amaral
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2011
Předmět:
Zdroj: Química Nova, Vol 34, Iss 9, Pp 1629-1633 (2011)
Druh dokumentu: article
ISSN: 1678-7064
0100-4042
DOI: 10.1590/S0100-40422011000900023
Popis: This works describes the use of experimental design and surface response methodology for optimization of saponin extraction from Ampelozizyphus amazonicus. For this purpose, a method employing extraction based on maceration assisted by ultrasound technique was utilized. The following factors were studied: extraction length of time and solvent composition. The total saponin was determined by using a gravimetric method and the results expressed by their relative proportion to total crude extract. For the specific condition, 60% hydro-alcoholic solution and 18 minutes extraction length of time has shown the best results. This method can be useful for extraction of substances with biological importance
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