The Effects of a Single Freeze-Thaw Cycle on Concentrations of Nutritional, Noncommunicable Disease, and Inflammatory Biomarkers in Serum Samples

Autor: Ransi Ann Abraham, Garima Rana, Praween K. Agrawal, Robert Johnston, Avina Sarna, Sowmya Ramesh, Rajib Acharya, Nizamuddin Khan, Akash Porwal, Sucheta Banerjee Kurundkar, Arvind Pandey, Raghu Pullakhandam, Krishnapillai Madhavan Nair, Geeta Trilok Kumar, HPS Sachdev, Umesh Kapil, Sila Deb, Arjan de Wagt, Ajay Khera, Lakshmy Ramakrishnan
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Journal of Laboratory Physicians, Vol 13, Iss 01, Pp 006-013 (2021)
Druh dokumentu: article
ISSN: 0974-2727
0974-7826
DOI: 10.1055/s-0041-1726575
Popis: Background The stability of biological samples is vital for reliable measurements of biomarkers in large-scale survey settings, which may be affected by freeze-thaw procedures. We examined the effect of a single freeze-thaw cycle on 13 nutritional, noncommunicable diseases (NCD), and inflammatory bioanalytes in serum samples. Method Blood samples were collected from 70 subjects centrifuged after 30 minutes and aliquoted immediately. After a baseline analysis of the analytes, the samples were stored at − 70°C for 1 month and reanalyzed for all the parameters. Mean percentage differences between baseline (fresh blood) and freeze-thaw concentrations were calculated using paired sample t-tests and evaluated according to total allowable error (TEa) limits (desirable bias). Results Freeze-thaw concentrations differed significantly (p < 0.05) from baseline concentrations for soluble transferrin receptor (sTfR) (− 5.49%), vitamin D (− 12.51%), vitamin B12 (− 3.74%), plasma glucose (1.93%), C-reactive protein (CRP) (3.45%), high-density lipoprotein (HDL) (7.98%), and cholesterol (9.76%), but they were within respective TEa limits. Low-density lipoprotein (LDL) (− 0.67%), creatinine (0.94%), albumin (0.87%), total protein (1.00%), ferritin (− 0.58%), and triglycerides (TAG) (2.82%) concentrations remained stable following the freeze-thaw cycle. In conclusion, single freeze-thaw cycle of the biomarkers in serum/plasma samples after storage at − 70°C for 1 month had minimal effect on stability of the studied analytes, and the changes in concentration were within acceptable limit for all analytes.
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