| Autor: |
Iolanda Vilela von Pinho, João Cândido de Souza, Renato Coelho de Castro Vasconcellos, Douglas Pelegrini Vaz-Tostes, Danielle Rezende Vilela, Wilson Vicente Souza Pereira |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Journal of Seed Science, Vol 46 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2317-1545 |
| DOI: |
10.1590/2317-1545v46282636 |
| Popis: |
ABSTRACT: Faster phenotyping tools are crucial for field progeny selection. We conducted research comparing two water deficit simulation methods on contrasting cultivars for water deficit tolerance. In a first step, we conducted two experiments: one for seed germination in sand at 10% and 70% water retention capacity, being analyzed seedling emergence and growth; other for seed germination in stress simulation by moistening the germination paper with PEG solution at -0.6 MPa, being analyzed seed germination. These experiments were used to distinguish characteristics of two maize lineages with different response to drought, being 57 - sensitive and 91 - tolerant. After that, we produced hybrid progenies from these lines at generations F1, F2, and F2:3 and applied the stress simulation by moistening the germination paper with -0.6 MPa of PEG solution. The seedling size was analyzed trough image analysis by the GroundEye® system. We could distinguish both lines and its hybrids through the stress simulation and image analysis. The results indicate that maize cultivars can be phenotyped for water deficit tolerance either 5 days postsowing through stress simulation or via image analysis of root length from seedlings germinated under -0.6 MPa. This method provides faster, more accurate, and more cost-effective methods for assessing water deficit tolerance in maize cultivars. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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