Sustainable treatment of nitrate-containing wastewater by an autotrophic hydrogen-oxidizing bacterium

Autor: Yi-Zhen Chen, Li-Juan Zhang, Ling-Yun Ding, Yao-Yu Zhang, Xi-Song Wang, Xue-Jiao Qiao, Bao-Zhu Pan, Zhi-Wu Wang, Nan Xu, Hu-Chun Tao
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Environmental Science and Ecotechnology, Vol 9, Iss , Pp 100146- (2022)
Druh dokumentu: article
ISSN: 2666-4984
DOI: 10.1016/j.ese.2022.100146
Popis: Bacteria are key denitrifiers in the reduction of nitrate (NO3−-N), which is a contaminant in wastewater treatment plants (WWTPs). They can also produce carbon dioxide (CO2) and nitrous oxide (N2O). In this study, the autotrophic hydrogen-oxidizing bacterium Rhodoblastus sp. TH20 was isolated for sustainable treatment of NO3−-N in wastewater. Efficient removal of NO3−-N and recovery of biomass nitrogen were achieved. Up to 99% of NO3−-N was removed without accumulation of nitrite and N2O, consuming CO2 of 3.25 mol for each mole of NO3−-N removed. The overall removal rate of NO3−-N reached 1.1 mg L−1 h−1 with a biomass content of approximately 0.71 g L−1 within 72 h. TH20 participated in NO3−-N assimilation and aerobic denitrification. Results from 15N-labeled-nitrate test indicated that removed NO3−-N was assimilated into organic nitrogen, showing an assimilation efficiency of 58%. Seventeen amino acids were detected, accounting for 43% of the biomass. Nitrogen loss through aerobic denitrification was only approximately 42% of total nitrogen. This study suggests that TH20 can be applied in WWTP facilities for water purification and production of valuable biomass to mitigate CO2 and N2O emissions.
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