Identification of Chlamydia trachomatis genotypes in newborns with respiratory distress

Autor: Melissa D. González-Fernández, Marco A. Escarcega-Tame, Marcela López-Hurtado, Verónica R. Flores-Salazar, Marcos R. Escobedo-Guerra, Silvia Giono-Cerezo, Fernando M. Guerra-Infante
Jazyk: Spanish; Castilian
Rok vydání: 2023
Předmět:
Zdroj: Anales de Pediatría (English Edition), Vol 98, Iss 6, Pp 436-445 (2023)
Druh dokumentu: article
ISSN: 2341-2879
DOI: 10.1016/j.anpede.2023.04.010
Popis: Introduction: One hundred thirty million Chlamydia trachomatis infections are reported worldwide each year. Nineteen serotypes of this pathogen can cause infection in pregnant women and neonates. The distribution of these genotypes in newborns with respiratory infections in Mexico is unknown. Material and methods: We tested 1062 bronchial lavage samples from neonates with respiratory distress syndrome for Chlamydia infection. The diagnosis of Chlamydia was made by plasmid detection with an in-house PCR assay, and genotypes were identified using a PCR-RFLP assay for the ompA gene. Results: The genotyping of 40 strains identified 14 as I/Ia (35%), 13 as E (32.5%), 7 as D (17.5%), 5 as F (12.5%), and 1 as L2 (2.5%). The relative risk analysis showed that genotype D was associated with neonatal sepsis (RR, 5.83; 95% confidence interval [CI], 1.51–25.985; P
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