Comprehensive virome analysis of the viral spectrum in paediatric patients diagnosed with Mycoplasma pneumoniae pneumonia

Autor: Qiong Guo, Lili Li, Chao Wang, Yiman Huang, Fenlian Ma, Shanshan Cong, Jingjing Tan, Lihong Yao, Aijun Chen, Lishu Zheng
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Virology Journal, Vol 19, Iss 1, Pp 1-10 (2022)
Druh dokumentu: article
ISSN: 1743-422X
DOI: 10.1186/s12985-022-01914-y
Popis: Abstract Background Among hospitalized children suffering from community-acquired pneumonia, Mycoplasma pneumoniae (MP) is one of the most common pathogens. MP often exists as a co-infection with bacteria or viruses, which can exacerbate the clinical symptoms. We investigated the pathogen spectrum in MP-positive and MP-negative samples from hospitalized children with respiratory tract infections in Beijing, China. Method This study included 1038 samples of nasopharyngeal aspirates obtained between April, 2017 and March, 2018 from hospitalized children under 6 years of age with respiratory tract infections. To explore the impact of MP infection on the composition of the pathogen spectrum, 185 nasopharyngeal aspirates (83 MP-positive/102 MP-negative) were randomly selected for next-generation sequencing and comprehensive metagenomics analysis. Real-time PCR was used to detect and verify common respiratory viruses. Results Of the 1038 samples, 454 (43.7%) were infected with MP. In children
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