| Autor: |
Olfat G. Shaker, Amina M. Alnoury, Gehan A. Hegazy, Hemmat E. El Haddad, Safaa Sayed, Ahmed Hamdy |
| Jazyk: |
English<br />Spanish; Castilian<br />Portuguese |
| Předmět: |
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| Zdroj: |
Revista Brasileira de Reumatologia, Vol 56, Iss 5, Pp 414-420 |
| Druh dokumentu: |
article |
| ISSN: |
1809-4570 |
| DOI: |
10.1016/j.rbre.2016.04.002 |
| Popis: |
ABSTRACT Background: Rheumatoid arthritis is a widely prevalent autoimmune disorder with suggested genetic predisposition. Objectives: The aim of this study is to detect the pattern of genetic polymorphism of methylene tetrahydrofolate reductase (MTHFR C677 T and A1298 C), transforming growth factor-β1 (TGF-β1 T869 C) and lymphotoxin-α (LT-α A252G) in patients having rheumatoid arthritis and correlate these patterns to disease activity and serum levels of tumor necrosis factor-alpha (TNF-α), B-Cell Activating Factor (BAFF), and osteopontin. Methods: A total of 194 subjects, 90 controls and 104 patients with rheumatoid arthritis were genotyped for MTHFR C677 T and A1298 C, TGF-β1 T869 C and LT-α A252G polymorphisms using a methodology based on PCR-RFLP. Also serum levels of TNF-α, osteopontin and BAFF were measured by ELISA kits. Results: The CT genotype and T allele of MTHFR C677 T and GG genotype and G allele of LT-α A252G are associated with the risk of RA and with higher levels of the pro-inflammatory cytokine, TNF-α in patients with rheumatoid arthritis. Conclusion: Our findings suggest that there is association between MTHFR C677 T and LT-α A252G genes polymorphisms and increased risk of RA in this sample of Egyptian population. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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