| Autor: |
Guixia Hao, Marion O'Connor, Weitian Liu, Wendell L. Roelofs |
| Jazyk: |
angličtina |
| Rok vydání: |
2002 |
| Předmět: |
|
| Zdroj: |
Journal of Insect Science, Vol 2, p 26 (2002) |
| Druh dokumentu: |
article |
| ISSN: |
1536-2442 |
| Popis: |
A delta11-desaturase gene was cloned from the sex pheromone gland of the obliquebanded leafroller moth, Choristoneura rosaceana. The desaturase cDNA sequence spans 1300 nucleotides with an open reading frame encoding a 335 amino-acid protein, which has 81% identity to a Z/E11-desaturase of the redbanded leafroller moth, Argyrotaenia velutinana. A functional assay with a pYES2 yeast expression system demonstrated that the delta11-desaturase exhibits unusual substrate and stereospecificities in producing a Z/E11 mixture (7:1) of only C14 acids. A metabolic Z9-desaturase also was cloned from fat body of this species, and proved to be in the class that produces more Z9-16:Acid than Z9-18:Acid. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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