| Popis: |
Abstract Background Balangu (Lallemantia royleana) belongs to Lamiaceae and is a medicine used in Iranian traditional and folklore medicine in the treatment of various nervous, hepatic, and renal diseases. Methods In this study, the influence of molecular weight (MW) was measured on antioxidant and antimicrobial activities of Balangu seed gum fractions. Firstly, Balangu seed gum was fractionated by precipitation method using ethanol on the basis of MW. Two fractions called precipitate (PER) Balangu and supernatant (SUPER) Balangu were obtained as the highest and lowest MW fractions, respectively. Monosaccharide composition was measured by GC-MS. The antioxidant activity was measured by two methods, DPPH and FRAP assay. The antibacterial activities were screened against two Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram-negative bacteria (Salmonella enterica and Escherichia coli) by minimum inhibitory and bactericidal concentration (MIC and MBC), disc, and well diffusion method. Results The results showed that the total phenol contents of Balangu, PER-Balangu, and SUPER-Balangu were 76.28 ± 1.41, 43.56 ± 1.72, and 89.46 ± 1.38 μg gallic acid equivalent/mg sample, respectively. Balangu and its fractions were composed of galactose, glucose, arabinose, rhamnose, and xylose. The IC50 values of Balangu, PER-Balangu, SUPER-Balangu, and BHT in DPPH assay were 0.46, 0.65, 0.38 and 0.31 mg/ml, respectively. In addition, the reducing power of Balangu, PER-Balangu, SUPER-Balangu, and BHT were 534.92, 431.80, 636.36, and 772.72 (mM Fe+2/g), respectively. Antibacterial test results showed, that for SUPER-Balangu, the minimum inhibitory concentration and the minimum bactericidal concentration against Staphylococcus were 3.125 mg/mL and 6.25 mg/mL. Conclusions SUPER-Balangu has a great potential as a natural food preservative, antibacterial, and antioxidant agent. |
