Small, Stable Shuttle Vectors Based on the Minimal pVS1 Replicon for Use in Gram-Negative, Plant-Associated Bacteria
Autor: | Stephan Heeb, Yoshifumi Itoh, Takayuki Nishijyo, Ursula Schnider, Christoph Keel, Julie Wade, Ultan Walsh, Fergal O'Gara, Dieter Haas |
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Jazyk: | angličtina |
Rok vydání: | 2000 |
Předmět: | |
Zdroj: | Molecular Plant-Microbe Interactions, Vol 13, Iss 2, Pp 232-237 (2000) |
Druh dokumentu: | article |
ISSN: | 1943-7706 0894-0282 |
DOI: | 10.1094/MPMI.2000.13.2.232 |
Popis: | The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month. |
Databáze: | Directory of Open Access Journals |
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