Small, Stable Shuttle Vectors Based on the Minimal pVS1 Replicon for Use in Gram-Negative, Plant-Associated Bacteria

Autor: Stephan Heeb, Yoshifumi Itoh, Takayuki Nishijyo, Ursula Schnider, Christoph Keel, Julie Wade, Ultan Walsh, Fergal O'Gara, Dieter Haas
Jazyk: angličtina
Rok vydání: 2000
Předmět:
Zdroj: Molecular Plant-Microbe Interactions, Vol 13, Iss 2, Pp 232-237 (2000)
Druh dokumentu: article
ISSN: 1943-7706
0894-0282
DOI: 10.1094/MPMI.2000.13.2.232
Popis: The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.
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