Polyphyllin I suppressed the apoptosis of intervertebral disc nucleus pulposus cells induced by IL-1β by miR-503-5p/Bcl-2 axis

Autor:	Lei Yuan, Hui Miao, Heng Ding, Fan Zhang, Zhen-kai Lou, Xing-Guo Li
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	Polyphyllin I IL-1β NPCs apoptosis miRNA-503-5p Bcl-2 Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935
Zdroj:	Journal of Orthopaedic Surgery and Research, Vol 18, Iss 1, Pp 1-10 (2023)
Druh dokumentu:	article
ISSN:	1749-799X
DOI:	10.1186/s13018-023-03947-7
Popis:	Abstract Background There are no studies that have shown the role and underlying mechanism of Polyphyllin I (PPI)-mediated anti-apoptosis activity in nucleus pulposus cells (NPCs). The research aimed to evaluate the effects of PPI in interleukin (IL)-1β-induced NPCs apoptosis in vitro. Methods Cell Counting Kit-8 (CCK-8) assay was used to detect cell viability, and cell apoptosis was evaluated by double-stained flow cytometry (FITC Annexin V/PI). The expression of miR-503-5p was quantified by real-time quantitative PCR (qRT-PCR), and the expression of Bcl-2, Bax, and cleaved caspase-3 was quantified by Western blot. Dual-luciferase reporter gene assay was used to detect the targeting relationship between miR-503-5p and Bcl-2. Results PPI at 40 μg·mL−1 markedly promoted the viability of NPCs (P
Databáze:	Directory of Open Access Journals
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