Exogenous Application of Polycationic Nanobactericide on Tomato Plants Reduces the Candidatus Liberibacter Solanacearum Infection

Autor: Adela Nazareth García-Sánchez, Roberto Yáñez-Macias, José Luis Hernández-Flores, Ariel Álvarez-Morales, José Humberto Valenzuela-Soto, Carlos Guerrero-Sanchez, Ramiro Guerrero-Santos
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Plants, Vol 10, Iss 10, p 2096 (2021)
Druh dokumentu: article
ISSN: 2223-7747
DOI: 10.3390/plants10102096
Popis: Candidatus Liberibacter solanacearum (CaLso) is associated with diseases in tomato crops and transmitted by the tomato psyllid Bactericera cockerelli. A polymeric water-dispersible nanobactericide (PNB) was evaluated against CaLso as a different alternative. PNB is a well-defined polycationic diblock copolymer designed to permeate into the vascular system of plants. Its assessment under greenhouse conditions was carried out with tomato plants previously infected with CaLso. Using a concentration as low as 1.0 mg L−1, a small but significant reduction in the bacterial load was observed by real-time qPCR. Thus, to achieve an ecologically friendly dosage and set an optimum treatment protocol, we performed experiments to determine the effective concentration of PNB to reduce ~65% of the initial bacterial load. In a first bioassay, a 40- or 70-fold increase was used to reach that objective. At this concentration level, other bioassays were explored to determine the effect as a function of time. Surprisingly, a real reduction in the symptoms was observed after three weeks, and there was a significant decrease in the bacterial load level (~98%) compared to the untreated control plants. During this period, flowering and formation of tomato fruits were observed in plants treated with PNB.
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