Detection of polyomavirus microRNA-5p expression in saliva shortly after kidney transplantation

Autor: Ana Carolina Mamana, Maria Stincarelli, Dmitry José De Santana Sarmento, Alexandre Mendes Batista, Tânia Regina Tozetto-Mendoza, Marina Gallottini, José Osmar Medina De Abreu Pestana, Paulo Henrique Braz-Silva, Simone Giannecchini
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Journal of Oral Microbiology, Vol 13, Iss 1 (2021)
Druh dokumentu: article
ISSN: 2000-2297
20002297
DOI: 10.1080/20002297.2021.1898838
Popis: Background: MicroRNAs (miRNAs) of polyomavirus (PyV) are present in several biological fluids and are suggested to be relevant viral factors for monitoring its persistence. Aim: To evaluate the effect of an immunosuppressive regimen on the status of PyV-miRNA-5p in the oral cavity. Materials and Methods: The JCPyV, BKPyV, MCPyV miRNA-5p were investigated in paired saliva and plasma samples obtained from 23 patients before and shortly after renal-transplantation by using real-time RT-PCR. Results: Overall, within a short-time after transplantation, patients exhibited decreased numbers of leukocyte and lymphocyte as well as low levels of creatinine. During the clinical management of the patients, a significant amount of saliva samples were positive for JCPyV and BKPyV miRNA-5p (range: 26%-91%) compared to paired plasma samples (range: 9%-35%). Among the two polyomaviruses showing positive expression of miRNA-5p, BKPyV presented the highest positivity in saliva (91%) and MCPyV-miRNA-5p was constantly negative in both saliva and plasma samples. Compared to the time before transplantation, a significant reduction in the expression of JCPyV-miRNA-5p was observed in saliva samples obtained after transplantation. Conclusions: Altogether, these data suggest that additional investigations of polyomavirus miRNA-5p in saliva should be performed shortly after renal-transplantation to evaluate the potential role in early viral reactivation.
Databáze: Directory of Open Access Journals
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