Evaluation of methylenetetrahydrofolate reductase and S-adenosyl-methionine level in male infertility: A case-control study

Autor: Khadijeh Baranizadeh, Maryam Bahmanzadeh, Heidar Tavilani, Tayebeh Ghiasvand, Iraj Amiri, Mahnaz Yavangi, Gholamreza Shafiee
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: International Journal of Reproductive BioMedicine, Vol 20, Iss 4, Pp 299-306 (2022)
Druh dokumentu: article
ISSN: 2476-4108
2476-3772
DOI: 10.18502/ijrm.v20i4.10902
Popis: Abstract Background: Methylenetetrahydrofolate reductase enzyme (MTHFR) plays a key role in regulating folate balance, converting homocysteine to methionine, and producing s-adenosylmethionine (SAM) that plays a role in the methylation process. Objective: This study aimed to determine MTHFR activity and SAM level in men with normozoospermia and oligozoospermia. Materials and Methods: 30 oligozoospermic and 30 normozoospermic men as controls were enrolled in this case-control study. Semen analysis was conducted according to the world health organization criteria. All semen samples were collected after 3-5 days of sexual abstinence. The sperms were evaluated by sperm test video software. All subjects SAM level was measured by enzyme-linked immunosorbent assay kit, and MTHFR were measured manually. Results: 2 groups had a significant difference in sperm morphology (p = 0.02), concentration (p = 0.02) and motility (p = 0.03). The MTHFR activity in normozoospermic and oligozoospermic groups had significantly differences (p = 0.01). The level of SAM in the semen of oligozoospermic men was statistically lower than normozoospermic men (p = 0.03). Also, there was a positive association between MTHFR enzyme activity and SAM level in the normozoospermia group (p = 0.02, β = 0.67) and oligozoospermia group (p = 0.03, β = 0.54). Conclusion: MTHFR activity and SAM concentration were statistically lower in oligozoospermia men. It seems they can affect sperm concentration, morphology, and motility.
Databáze: Directory of Open Access Journals
Externí odkaz: