| Autor: |
Raoul A Droeser, Christian Hirt, Serenella Eppenberger-Castori, Inti Zlobec, Carsten T Viehl, Daniel M Frey, Christian A Nebiker, Raffaele Rosso, Markus Zuber, Francesca Amicarella, Giandomenica Iezzi, Giuseppe Sconocchia, Michael Heberer, Alessandro Lugli, Luigi Tornillo, Daniel Oertli, Luigi Terracciano, Giulio C Spagnoli |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
PLoS ONE, Vol 8, Iss 5, p e64814 (2013) |
| Druh dokumentu: |
article |
| ISSN: |
1932-6203 |
| DOI: |
10.1371/journal.pone.0064814 |
| Popis: |
BACKGROUND: Colorectal cancer (CRC) infiltration by adaptive immune system cells correlates with favorable prognosis. The role of the innate immune system is still debated. Here we addressed the prognostic impact of CRC infiltration by neutrophil granulocytes (NG). METHODS: A TMA including healthy mucosa and clinically annotated CRC specimens (n = 1491) was stained with MPO and CD15 specific antibodies. MPO+ and CD15+ positive immune cells were counted by three independent observers. Phenotypic profiles of CRC infiltrating MPO+ and CD15+ cells were validated by flow cytometry on cell suspensions derived from enzymatically digested surgical specimens. Survival analysis was performed by splitting randomized data in training and validation subsets. RESULTS: MPO+ and CD15+ cell infiltration were significantly correlated (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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