'Baixadeiros' horses: prevalence of anti-Trypanosoma spp. and anti-Leishmania spp. antibodies
Autor: | Fernanda Pinto Ferreira, Eloiza Teles Caldart, Danilo Rodrigues Barros Brito, Daniel Praseres Chaves, João Luis Garcia, Italmar Teodorico Navarro |
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Jazyk: | English<br />Portuguese |
Rok vydání: | 2018 |
Předmět: | |
Zdroj: | Ciência Animal Brasileira, Vol 19, Pp 1-8 (2018) |
Druh dokumentu: | article |
ISSN: | 1518-2797 1809-6891 19051522 |
DOI: | 10.1590/cab19051522 |
Popis: | A total of 138 horse blood samples were collected from 2012 to 2013 for the subsequent separation of serum collection. For detection of anti-Leishmania spp. and anti-Trypanosoma spp. antibodies, indirect immunofluorescence reactions and immunoenzymatic assays were performed. Samples with titers ≥40 were considered positive in the indirect immunofluorescence (IFAT), and the cut off for the enzyme immunoassay (ELISA) was calculated with three standard deviations. When tested for Trypanosoma spp., 50.37% (68/135) of the samples were ELISA reagents, 18.84% (8/136) were positive for IFAT and 2.89% (4/138) for both tests. A significant statistical association was observed for the municipality (p = 0.013) and collection period (p = 0.042) in the ELISA. When tested for Leishmania spp., 25.4% (35/138) of the samples were ELISA reagents, 13.00% (18/138) were positive for the IFAT and 4.34% (6/138) were positive for two tests, there were no variables associated with seropositivity. A statistical association (p = 0.0034) was observed between the presence of anti-Trypanosoma spp. and anti-Leishmania spp. antibodies when the ELISA was used. The results suggest that this horse breed is in contact with leishmaniasis and trypanosomiasis, which demonstrates the need for a more accurate investigation into the real role of horses in these diseases to assist in disease control measures.Keywords: Leishmaniasis; Trypanosomiasis; Equines; Serology |
Databáze: | Directory of Open Access Journals |
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