| Autor: |
Daniel J. Sargent, Paulina Kuchta, Elena Lopez Girona, Hailong Zhang, Thomas M. Davis, Jean-Marc Celton, Annalisa Marchese, Malgorzata Korbin, Kevin M. Folta, Vladimir Shulaev, David W. Simpson |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
The Plant Genome, Vol 4, Iss 3, Pp 165-177 (2011) |
| Druh dokumentu: |
article |
| ISSN: |
1940-3372 |
| DOI: |
10.3835/plantgenome2011.05.0014 |
| Popis: |
The genome sequence of the woodland strawberry ( L.) is an important resource providing a reference for comparative genomics studies and future sequenced rosaceous species and has great utility as a model for the development of markers for mapping in the cultivated strawberry Duchesne ex Rozier. A set of 152 microsatellite simple sequence repeat (SSR) primer pairs was developed and mapped, along with 42 previously published but unmapped SSRs, permitting the precise assignment of 28.2 Mbp of previously unanchored genome sequence scaffolds (13% of the genome sequence). The original ordering of sequence scaffolds was performed without a physical map, using predominantly SSR markers to order scaffolds via anchoring to a comprehensive linkage map. This report complements and expands resolution of the spp. reference map and refines the scaffold ordering of the genome sequence using newly devised tools. The results of this study provide two significant resources: (i) the concurrent validation of a substantial set of SSRs associated with these previously unmapped regions of the spp. genome and (ii) the precise placement of previously orphaned genomic sequence. Together, these resources improve the resolution and completeness of the strawberry genome sequence, making it a better resource for downstream studies in spp. and the family Rosaceae. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
Plný text