Stability-indicating RP-HPLC method for determination of beclomethasone dipropionate in nanocapsule suspensions
| Autor: | Janaíne Micheli Chassot, Luana Mota Ferreira, Felipe Pereira Gomes, Letícia Cruz, Leandro Tasso |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences, Vol 51, Iss 4, Pp 803-810 (2015) |
| Druh dokumentu: | article |
| ISSN: | 2175-9790 1984-8250 |
| DOI: | 10.1590/S1984-82502015000400006 |
| Popis: | abstract A simple stability-indicating RP-HPLC/UV method was validated for determination of beclomethasone dipropionate (BD) in nanocapsule suspensions. Chromatographic conditions consisted of a RP C18column (250 mm x 4.60 mm, 5 µm, 110 Å), using methanol and water (85:15 v/v) as mobile phase at 1.0 mL/min with UV detection at 254 nm. The calibration curve was found to be linear in the concentration range of 5.0-25.0 µg/mL with a correlation coefficient > 0.999. Precision was demonstrated by a relative standard deviation lower than 2.0%. Accuracy was assessed by the recovery test of BD from nanocapsules (98.03% to 100.35%). Specificity showed no interference from the components of nanocapsules or from the degradation products derived from acid, basic and photolytic conditions. In conclusion, the method is suitable to be applied to assay BD in bulk drug and in nanocapsules, and it can be employed to study stability and degradation kinetics. |
| Databáze: | Directory of Open Access Journals |
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