Enzymolysis Technology Optimization for Production of Antioxidant Peptides from Goat Milk Casein

Autor: Shu Guowei, Wang Zhuo, Chen Li, Zhang Qian, Xin Ni
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Acta Universitatis Cibiniensis. Series E: Food Technology, Vol 21, Iss 1, Pp 51-60 (2017)
Druh dokumentu: article
ISSN: 2344-150X
DOI: 10.1515/aucft-2017-0006
Popis: Antioxidant peptides can inhibit lipid peroxidation and scavenging free radicals, maintain the balance of free radicals, and against a variety of diseases. Response surface methodology was used to optimize process conditions for producing antioxidative peptides from goat’s milk casein hydrolysate with Alcalase. The results suggested that the optimal process parameters were: temperature at 62.5°C, pH 8.9, E/S ration at 2.5%, substrate concentration at 4.4% and hydrolysis time was 173min). Metal-chelating effect, superoxide anion radical scavenging activity and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity were shown to be 87.21±0.88%, 49.18±1.42% and 69.07±1.26% respectively under the optimal condition. The actual and predicated value were closely which indicated the optimized data fit well to model and the optimized parameters are reliable.
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