| Autor: |
Lijun ZHANG, Tiantian ZHAO, Jieqiong CHEN, Kangrong ZHONG, Yongjian GUAN, Rui LUO, Chunyan LIN, Haijun NAN, Yehui ZHANG |
| Jazyk: |
čínština |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Shipin gongye ke-ji, Vol 45, Iss 19, Pp 316-324 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1002-0306 |
| DOI: |
10.13386/j.issn1002-0306.2023100023 |
| Popis: |
This study was designed to explore the hepatoprotective effects of enzymatic degradation products derived from sturgeon roe on alcohol-damaged hepatocytes and to elucidate the underlying mechanisms. Utilizing sturgeon roe as the research material, the enzymatic process was optimized by focusing on key parameters such as enzymatic efficiency and in vitro activity. The protective effects on hepatocytes were systematically investigated by using the HepG2 cell model. Advanced computerized virtual screening technology was employed to identify potential bioactive peptides within the sturgeon roe. The findings indicated that the optimum enzymatic product of sturgeon roe was obtained under the conditions of 1:1 compounding of alkaline protease (1343 U/g) and trypsin (27 U/g) for 8 h, yielded a protein recovery of 49.30%±0.57%, a hydrolysis degree of 46.05%±0.92%, and ABTS+ free radical scavenging rate of 51.45%±0.66%. Notably, at a concentration of 5 mg/mL, this product significantly enhanced the activity of alcohol dehydrogenase (ADH) by 168.64%±1.42% (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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