Antifungal Ability of DNA-AgNCs under Molecular Crowding and Its Application in Citrus Preservation

Autor: Ning DU, Xu CHEN, Haiyan LIU, Wentao XU
Jazyk: čínština
Rok vydání: 2022
Předmět:
Zdroj: Shipin gongye ke-ji, Vol 43, Iss 24, Pp 347-354 (2022)
Druh dokumentu: article
ISSN: 1002-0306
DOI: 10.13386/j.issn1002-0306.2022030350
Popis: In order to investigate the effect of molecular crowding on the antifungal ability of DNA-AgNCs and its preservation effect on citrus, DNA sequence (5'-CCCCCCCCCCCC-3') was mixed with AgNO3 solution in this experiment. DNA-AgNCs was prepared by simulating molecular crowded environment with 50% Polyethylene Glycol (PEG) 200 solution. Taking DNA-AgNCs as the research object, using Penicillium expansum, Aspergillus flavus, Fusarium graminaerum and Fusarium moniliforme as the models, the inhibition rate of DNA-AgNCs on the germination of four kinds of spores was determined by spore counting method, and the Minimal Inhibitory Concentration (MIC) of DNA-AgNCs against four kinds of fungi was determined by microdilution method. DNA-AgNCs was added to starch-chitosan matrix to prepare composite films, and its effects on the mechanical properties, hydrophobicity and antibacterial properties of the films were explored through tensile test, water contact angle and inhibition zone test. The film was applied to citrus preservation in the form of coating film, and the weight loss rate, plaque diameter and sensory evaluation of citrus were recorded. The results showed that the inhibitory effect of molecular crowded DNA-AgNCs on spore germination was better than that of normal DNA-AgNCs (P
Databáze: Directory of Open Access Journals