Autor: |
Xiangfu Gu, Jiaqi Tang, Yue Zhao, Chuhong Su, Lingyu Xiao, Huiyu Luo, Yuguo Liu, Fei Xiong, Zhongdaixi Zheng, Junbin Chen, Longying Zha |
Jazyk: |
angličtina |
Rok vydání: |
2021 |
Předmět: |
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Zdroj: |
Journal of Functional Foods, Vol 87, Iss , Pp 104789- (2021) |
Druh dokumentu: |
article |
ISSN: |
1756-4646 |
DOI: |
10.1016/j.jff.2021.104789 |
Popis: |
The mechanisms of soyasaponin A1 (SS-A1) on antagonizing inflammation via regulating the lipid raft-mediated toll-like receptor 4 (TLR4) signaling are not completely understood. In this study, SS-A1 inhibited palmitic acid (PA)-induced recruitments of TLR4 and its adaptor molecules into lipid rafts in Raw264.7 macrophage cell line by using ultracentrifugation and confocal microscopy. SS-A1 also suppressed the PA-caused dimerization of TLR4 with and its adaptor molecules by applying immunoprecipitation and fluorescence resonance energy transfer. Meanwhile, SS-A1 modified the formation, clustering, and size of lipid rafts and decreased cholesterol in lipid rafts. Cholesterol replenishment abrogated SS-A1’s inhibition on the lipid rafts recruitment and dimerization of TLR4 and its adaptor molecules and its anti-inflammatory activity. Additionally, SS-A1 inhibited the mature sterol regulatory element-binding protein 2 and increases ATP-binding cassette transporter A1. Collectively, SS-A1 inhibits the lipid raft recruitment and dimerization of TLR4 and its adaptor molecules by maintaining cholesterol homeostasis in PA-stimulated inflammatory Raw264.7 macrophage cell line. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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