| Autor: |
Haowen Liu, Lei Li, Mia Krout, Seema Sheoran, Qihong Zhao, Jingyi Chen, Huisheng Liu, Janet E. Richmond, Zhitao Hu |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 2, Iss 3, Pp 100749- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2021.100749 |
| Popis: |
Summary: Release of neurotransmitters by synaptic vesicle exocytosis at presynaptic terminals is critical for neuronal communication within the nervous system. Electrophysiology and electron microscopy are powerful and complementary approaches used to evaluate the function of synaptic proteins in synaptic transmission. Here, we provide a protocol detailing the use of these two approaches at C. elegans neuromuscular junctions, including steps for worm picking and dissection, in vivo electrophysiological recording, and sample preparation for electron microscopy, followed by imaging and analysis.For complete details on the use and execution of this protocol, please refer to Liu et al. (2021) and Li et al. (2021). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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