| Autor: |
Ryuta Koyama, Rieko Muramatsu, Takuya Sasaki, Rie Kimura, Chihiro Ueyama, Makoto Tamura, Naohiro Tamura, Junya Ichikawa, Naoya Takahashi, Atsushi Usami, Maki K. Smith, Norio Matsuki, Yuji Ikegaya |
| Jazyk: |
angličtina |
| Rok vydání: |
2007 |
| Předmět: |
|
| Zdroj: |
Journal of Pharmacological Sciences, Vol 104, Iss 2, Pp 191-194 (2007) |
| Druh dokumentu: |
article |
| ISSN: |
1347-8613 |
| DOI: |
10.1254/jphs.SC0070119 |
| Popis: |
Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM® method. Keywords:: membrane-interface tissue culture, organotypic culture, hippocampal slice |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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