Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy
| Autor: | R. KAŇA, B. ŠEDIVÁ, O. PRÁŠIL |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Photosynthetica, Vol 61, Iss SPECIAL ISSUE 2023-2, Pp 483-491 (2023) |
| Druh dokumentu: | article |
| ISSN: | 0300-3604 1573-9058 |
| DOI: | 10.32615/ps.2023.043 |
| Popis: | The investigation of spatial heterogeneity within the thylakoid membrane (TM) proteins has gained increasing attention in photosynthetic research. The recent advances in live-cell imaging have allowed the identification of heterogeneous organisation of photosystems in small cyanobacterial cells. These sub-micrometre TM regions, termed microdomains in cyanobacteria, exhibit functional similarities with granal (Photosystem II dominant) and stromal (Photosystem I dominant) regions observed in TM of higher plants. This study delves into microdomain heterogeneity using super-resolution Airyscan-based microscopy enhancing resolution to approximately ~125 nm in x-y dimension. The new data reveal membrane areas rich in Photosystem I within the inner TM rings. Moreover, we identified analogous dynamics in the mobility of Photosystem II and phycobilisomes; countering earlier models that postulated differing mobility of these complexes. These novel findings thus hold significance for our understanding of photosynthesis regulation, particularly during state transitions. |
| Databáze: | Directory of Open Access Journals |
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