Role of neurotrophic factors in enhancing linear axonal growth of ganglionic sensory neurons in vitro
| Autor: | Michele Fornaro, Alessia Giovannelli, Angelica Foggetti, Luisa Muratori, Stefano Geuna, Giorgia Novajra, Isabelle Perroteau |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
brain-derived neurotrophic factor
directionality dorsal root ganglia explant nerve growth factor nerve regeneration neurite growth enhancement neurotrophic factors neurotrophin-3 sensory neurons tortuosity chinese library classification no. r453 r364 r741 Neurology. Diseases of the nervous system RC346-429 |
| Zdroj: | Neural Regeneration Research, Vol 15, Iss 9, Pp 1732-1739 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1673-5374 |
| DOI: | 10.4103/1673-5374.276338 |
| Popis: | Neurotrophins play a major role in the regulation of neuronal growth such as neurite sprouting or regeneration in response to nerve injuries. The role of nerve growth factor, neurotrophin-3, and brain-derived neurotrophic factor in maintaining the survival of peripheral neurons remains poorly understood. In regenerative medicine, different modalities have been investigated for the delivery of growth factors to the injured neurons, in search of a suitable system for clinical applications. This study was to investigate the influence of nerve growth factor, neurotrophin-3 and brain-derived neurotrophic factor on the growth of neurites using two in vitro models of dorsal root ganglia explants and dorsal root ganglia-derived primary cell dissociated cultures. Quantitative data showed that the total neurite length and tortuosity were differently influenced by trophic factors. Nerve growth factor and, indirectly, brain-derived neurotrophic factor stimulate the tortuous growth of sensory fibers and the formation of cell clusters. Neurotrophin-3, however, enhances neurite growth in terms of length and linearity allowing for a more organized and directed axonal elongation towards a peripheral target compared to the other growth factors. These findings could be of considerable importance for any clinical application of neurotrophic factors in peripheral nerve regeneration. Ethical approval was obtained from the Regione Piemonte Animal Ethics Committee ASLTO1 (file # 864/2016-PR) on September 14, 2016. |
| Databáze: | Directory of Open Access Journals |
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