Evaluation of dietary addition of 2 essential oils from Achillea moschata, or their components (bornyl acetate, camphor, and eucalyptol) on in vitro ruminal fermentation and microbial community composition

Autor: Stefania Colombini, Andrea Rota Graziosi, Pietro Parma, Marcello Iriti, Sara Vitalini, Chiara Sarnataro, Mauro Spanghero
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Animal Nutrition, Vol 7, Iss 1, Pp 224-231 (2021)
Druh dokumentu: article
ISSN: 2405-6545
DOI: 10.1016/j.aninu.2020.11.001
Popis: This study investigated the effects of 2 Achillea moschata essential oils extracted from plants collected in 2 different valleys of the Italian Alps and 3 pure compounds of oils — bornyl acetate (BOR), camphor (CAM), and eucalyptol (EUCA) — on in vitro ruminal fermentation and microbiota. An in vitro batch fermentation experiment (Exp. 1) tested the addition of all of the substances (2 essential oils and 3 compounds) in fermentation bottles (120 mL) at 48 h of incubation, whereas a subsequent in vitro continuous culture experiment (Exp. 2) evaluated the pure compounds added to the fermenters (2 L) for a longer incubation period (9 d). In both experiments, total mixed rations were incubated with the additives, and samples without additives were included as the control (CTR). Each treatment was tested in duplicate and was repeated in 3 and 2 fermentation runs in Exp. 1 and 2, respectively. Gas production (GP) in Exp. 1 was similar for all of the treatments, and short chain volatile fatty acid (SCFA) production was similar in both experiments except for a decrease of SCFA produced (P = 0.029) due to EUCA addition in Exp. 2. Compared to CTR, BOR and CAM reduced the valerate proportion (P = 0.04) in Exp. 1, and increased (P
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