Empowering Shotgun Mass Spectrometry with 2DE: A HepG2 Study

Autor:	Olga Kiseleva, Victor Zgoda, Stanislav Naryzhny, Ekaterina Poverennaya
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	proteome transcriptome proteoforms alternative splicing single amino acid polymorphisms post-translational modifications Biology (General) QH301-705.5 Chemistry QD1-999
Zdroj:	International Journal of Molecular Sciences, Vol 21, Iss 11, p 3813 (2020)
Druh dokumentu:	article
ISSN:	1422-0067 1661-6596
DOI:	10.3390/ijms21113813
Popis:	One of the major goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to catalog and annotate a myriad of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve a detailed and personalized understanding into proteomes, we suggest using a customized RNA-seq library of potential proteoforms, which includes aberrant variants specific to certain biological samples. Two-dimensional electrophoresis coupled with high-performance liquid chromatography allowed us to downgrade the difficulty of biological mixing following shotgun mass spectrometry. To benchmark the proposed pipeline, we examined heterogeneity of the HepG2 hepatoblastoma cell line proteome. Data are available via ProteomeXchange with identifier PXD018450.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/f695ade779a4456fab0102c62514d15e Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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