Flow Cytometry Applications in the Study of Immunological Lung Disorders
| Autor: | Esmaeil Mortaz, Hoda Gudarzi, Payam Tabarsi, Ian M Adcock, Mohamad Reza Masjedi, Hamid Reza Jamaati, Johan Garssen, Ali Akbar Velayati, Frank A Redegeld |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Iranian Journal of Allergy, Asthma and Immunology, Vol 14, Iss 1 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1735-1502 1735-5249 |
| Popis: | The use of flow cytometry in the clinical laboratory has grown substantially in the past decade. Flow cytometric analysis provides a rapid qualitative and quantitative description of multiple characteristics of individual cells. For example, it is possible to detect the cell size and granularity, aspects of DNA and RNA content and the presence of cell surface and nuclear markers which are used to characterize the phenotype of single cells. Flow cytometry has been used for the immunophenotyping of a variety of specimens including whole blood, bone marrow, serous cavity fluids, (cerebrospinal fluid) CSF, urine and all types of body fluids. The technique has also been applied to human bronchoalveolar lavage (BAL) fluid, peritoneal fluids and blood. In this review, we describe the current status of the application of flow cytometry as a diagnostic tool in various lung diseases. We focus on the analysis of BAL cell composition in chronic obstructive lung disease (COPD), asthma, lung cancer, sarcoidosis, tuberculosis and idiopathic eosinophilic pneumonia (IEP). |
| Databáze: | Directory of Open Access Journals |
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