Effect of deoxynivalenol and T-2 toxin in malt amylase activity
| Autor: | Jaqueline Garda-Buffon, Edlira Baraj, Eliana Badiale-Furlong |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Brazilian Archives of Biology and Technology, Vol 53, Iss 3, Pp 505-511 (2010) |
| Druh dokumentu: | article |
| ISSN: | 1516-8913 1678-4324 |
| DOI: | 10.1590/S1516-89132010000300002 |
| Popis: | The objective of this work was to evaluate the effect of DON and T-2 toxin levels on the malt amylase activity. The malt was contaminated in accordance with central composite design experiment with DON and T-2 toxin levels until 1000 ng/g. The activities of the enzymes were evaluated by Bernfeld method. The increase in T-2 toxin concentration inhibited the α-amylase activity. However, the increase of both toxins concentration caused inverse effect. The interaction between toxins indicated synergetic effect on the β-amylase activity. An increased activity occurred when the toxins contamination levels in malt were higher (1000ng/g malt). The trichothecenes interfered with the performance of aminolitic enzymes in the stage of malting, resulting in a significant model for enzymatic activity of β-amylase.O objetivo deste trabalho foi avaliar o efeito de deoxynivalenol e toxina T-2 na atividade de a e b-amilase em malte. A contaminação artificial foi de acordo com planejamento experimental completo nos níveis de 0 a 1000ng/g para cada micotoxina. O aumento da concentração da toxina T-2 inibiu a atividade da a-amilase. Entretanto, a coocorrência a altas concentrações das duas toxinas indicou efeito sinergistíco. Um aumento da atividade enzimática ocorreu na presença de altos níveis das micotoxinas avaliadas (1000ng/g de malte). Os tricotecenos interferiram na performance das enzimas aminolíticas na etapa de malteação, permitindo determinar um modelo preditivo para a atividade da β-amylase. |
| Databáze: | Directory of Open Access Journals |
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