Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles

Autor: Valéria da Silva Guimarães, Regina Lucia dos Santos Silva, Ricássio de Souza Barberino, Istefani Moreira Mota, Joisyleide Gonçalves Costa Pinto, Maria Lilian Gomes Loiola Torres, Naiane Darklei do Santos Silva, Mário Adriano Ávila Queiroz, Maria Helena Tavares de Matos, Alane Pains Oliveira do Monte
Jazyk: English<br />Portuguese
Rok vydání: 2023
Předmět:
Zdroj: Semina: Ciências Agrárias, Vol 44, Iss 6 (2023)
Druh dokumentu: article
ISSN: 1679-0359
1676-546X
DOI: 10.5433/1679-0359.2023v44n6p1991
Popis: This study was conducted to evaluate the effects of the acidified extract of M. oleifera leaves as a supplement into the base medium for in vitro culture of sheep isolated secondary follicles. Follicles were isolated and cultured for 12 days in α-MEM+(supplemented with bovine serum albumin, insulin, glutamine, hypoxanthine, transferrin, selenium, and ascorbic acid) with or without 0.1; 0.2 or 0.4 mg/ml of the acidified extract of M. oleifera. Follicle morphology, antral cavity formation, follicular and oocyte diameter, glutathione (GSH) concentration, mitochondrial activity and meiotic resumption were evaluated. After 12 days of culture, there was no significant difference among treatments in relation to follicular morphology, antral cavity formation, diameter and mitochondrial activity. Nevertheless, oocytes from follicles cultured in α-MEM+ showed greater GSH concentration than media containing M. oleifera extract. Furthermore, the concentration of 0.4 mg/ml M. oleifera extract significantly increased the percentage of fully grown oocyte (≥ 110 µm) when compared to the other treatments. In conclusion, the concentration of 0.4 mg/ml M. oleifera extract as a supplement of the culture medium, maintained the survival, and increased the percentage of fully grown oocytes.
Databáze: Directory of Open Access Journals
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