| Autor: |
Xulio Benítez, Elena G. Gonzalez, Jesus García, Paz Zúñiga, Fernando de laCalle, Carmen Cuevas |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Microbial Biotechnology, Vol 14, Iss 1, Pp 241-250 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
1751-7915 |
| DOI: |
10.1111/1751-7915.13679 |
| Popis: |
Summary The continued development of culturing technologies for the discovery of new molecules from marine microbes is of paramount importance for drug discovery. Coupled with this, the use of the high‐throughput approach shows promise for increasing the number of Gram‐negative and non‐filamentous bacteria cultures that can be surveyed, since they show a lower potential of bioactivity. In this work, we propose a new strategy of high‐throughput cultivation of bacteria inspired by a dilution‐to‐extinction (DTE) methodology for the isolation of, and screening for, new cytotoxic compound producing marine bacteria. A marine sponge tissue was directly used as inoculum and the results were compared with the data obtained through the direct plating isolation method. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC‐PCR) genomic fingerprinting indicated the isolation of four bioactive strains, three of them producers of a pederin‐like compound, and the fourth one able to synthesize a different compound, still unidentified, rendered by the DTE approach, in comparison with one bioactive strain identified through the plating method. Analyses based on the 16S rRNA gene data showed the existence of two different species belonging to the genus Labrenzia. The efficiency and diversity ratio in the number of isolates and compounds are discussed. In view of the results, the proposed DTE approach proved to be efficient for the isolation of new cytotoxic compounds of marine origin and pave the way for future potential applications. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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