| Autor: |
Andrea Pecora, Darío A Malacari, María S Pérez Aguirreburualde, Demian Bellido, José M Escribano, María J. Dus Santos, Andrés Wigdorovitz |
| Jazyk: |
English<br />Spanish; Castilian |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Revista Argentina de Microbiología, Vol 47, Iss 1, Pp 4-8 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
1851-7617 |
| DOI: |
10.1016/j.ram.2014.12.004 |
| Popis: |
Bovine viral diarrhea virus (BVDV) is an important cause of economic losses worldwide. E2 is an immunodominant protein and a promising candidate to develop subunit vaccines. To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody named APCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed in the baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2 vaccine was the best one to evoke a humoral response, and for this reason, it was selected for a cattle vaccination experiment. All the bovines immunized with 1.5 µg of APCH-tE2 developed high levels of neutralizing antibodies against BVDV up to a year post-immunization, demonstrating its significant potential as a subunit vaccine. This novel vaccine is undergoing scale-up and was transferred to the private sector. Nowadays, it is being evaluated for registration as the first Argentinean subunit vaccine for cattle. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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