First identification and molecular subtyping of Blastocystis sp. in zoo animals in southwestern China

Autor: Lei Deng, Jingxin Yao, Shanyu Chen, Tingmei He, Yijun Chai, Ziyao Zhou, Xiaogang Shi, Haifeng Liu, Zhijun Zhong, Hualin Fu, Guangneng Peng
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Parasites & Vectors, Vol 14, Iss 1, Pp 1-11 (2021)
Druh dokumentu: article
ISSN: 1756-3305
DOI: 10.1186/s13071-020-04515-2
Popis: Abstract Background Blastocystis sp. is an anaerobic protozoan that parasitizes many animal hosts and the human gastrointestinal tract, and its pathogenicity is controversial. Captive wildlife may be potential reservoirs for human infection with Blastocystis sp. The present study was performed to investigate the prevalence and subtype distribution of Blastocystis sp. in zoo animals in Sichuan Province, southwestern China. Methods A total of 420 fresh fecal samples were collected from 54 captive wildlife species in four zoos in southwestern China between June 2017 and September 2019. The prevalence and subtype (ST) genetic characteristics of Blastocystis sp. were determined by PCR amplification of the barcode region of the SSU rRNA gene and phylogenetic analysis. Results Overall, 15.7% (66/420) of the animal samples and 20.7% (14/54) of the species tested were shown to be infected with Blastocystis sp. The highest prevalence of Blastocystis sp. was found in Panzhihua Zoo (24.3%), which was significantly higher than that in Chengdu Zoo (6.9%), and Xichang Zoo (2.9%) (P
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