| Autor: |
Victor J. Pai, Calvin J. Lau, Almudena Garcia-Ruiz, Cynthia Donaldson, Joan M. Vaughan, Brendan Miller, Eduardo V. De Souza, Antonio M. Pinto, Jolene Diedrich, Narender R. Gavva, Shan Yu, Christopher DeBoever, Shane R. Horman, Alan Saghatelian |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
BMC Genomics, Vol 25, Iss 1, Pp 1-21 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1471-2164 |
| DOI: |
10.1186/s12864-024-10948-1 |
| Popis: |
Abstract Background Recent analysis of the human proteome via proteogenomics and ribosome profiling of the transcriptome revealed the existence of thousands of previously unannotated microprotein-coding small open reading frames (smORFs). Most functional microproteins were chosen for characterization because of their evolutionary conservation. However, one example of a non-conserved immunomodulatory microprotein in mice suggests that strict sequence conservation misses some intriguing microproteins. Results We examine the ability of gene regulation to identify human microproteins with potential roles in inflammation or fibrosis of the intestine. To do this, we collected ribosome profiling data of intestinal cell lines and peripheral blood mononuclear cells and used gene expression of microprotein-encoding transcripts to identify strongly regulated microproteins, including several examples of microproteins that are only conserved with primates. Conclusion This approach reveals a number of new microproteins worthy of additional functional characterization and provides a dataset that can be queried in different ways to find additional gut microproteins of interest. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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