NADP-malic Enzyme OsNADP-ME2 Modulates Plant Height Involving in Gibberellin Signaling in Rice

Autor: Bing Li, Xiaolong Zhou, Wei Yao, Jinjun Lin, Xiaowen Ding, Qianru Chen, Hao Huang, Wenfeng Chen, Xilai Huang, Sujun Pan, Yinghui Xiao, Jianfeng Liu, Xionglun Liu, Jinling Liu
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Rice, Vol 17, Iss 1, Pp 1-10 (2024)
Druh dokumentu: article
ISSN: 1939-8425
1939-8433
DOI: 10.1186/s12284-024-00729-5
Popis: Abstract Plants NADP-malic enzymes (NADP-MEs) act as a class of oxidative decarboxylase to mediate malic acid metabolism in organisms. Despite NADP-MEs have been demonstrated to play pivotal roles in regulating diverse biological processes, the role of NADP-MEs involving in plant growth and development remains rarely known. Here, we characterized the function of rice cytosolic OsNADP-ME2 in regulating plant height. The results showed that RNAi silencing and knock-out of OsNADP-ME2 in rice results in a dwarf plant structure, associating with significant expression inhibition of genes involving in phytohormone Gibberellin (GA) biosynthesis and signaling transduction, but with up-regulation for the expression of GA signaling suppressor SLR1. The accumulation of major bioactive GA1, GA4 and GA7 are evidently altered in RNAi lines, and exogenous GA treatment compromises the dwarf phenotype of OsNADP-ME2 RNAi lines. RNAi silencing of OsNADP-ME2 also causes the reduction of NADP-ME activity associating with decreased production of pyruvate. Thus, our data revealed a novel function of plant NADP-MEs in modulation of rice plant height through regulating bioactive GAs accumulation and GA signaling, and provided a valuable gene resource for rice plant architecture improvement.
Databáze: Directory of Open Access Journals
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