| Autor: |
Li Yongjie, Sun Yuxiang, Lin Hongchun, Li Canming, Shang Hongli, Peng Hui |
| Jazyk: |
čínština |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Xin yixue, Vol 53, Iss 4, Pp 292-296 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
0253-9802 |
| DOI: |
10.3969/j.issn.0253-9802.2022.04.013 |
| Popis: |
Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis （PD） patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcriptome data of PD effluent cells from 10 PD patients （6 cases in the short-term PD group and 4 cases in the long-term PD group） obtained in our previous study, and the data of T lymphocytes were extracted to analyze the differences between two groups, and pathway enrichment analysis was also performed. At the same time, complete blood count data of 23 PD patients before and after PD were collected, and the differences in immune cell composition were compared before and after PD. Results The proportion of T lymphocytes to the total number of immune cells in the PD effluent was increased significantly with the prolongation of PD time （P < 0.05）. Differential analysis showed that TGF-β1 and other fibrosis-related signaling pathways were enriched in peritoneal T lymphocytes in the long-term PD group. Compared with the complete blood count before dialysis, the lymphocyte count in both short-term and long-term PD groups was significantly increased, but the proportion was initially increased, and subsequently decreased. Conclusions Peritoneal fibrosis caused by long-term PD treatment is closely associated with T lymphocytes. And the changes of T lymphocytes in PD effluent may be jointly caused by the peritoneal environment and alternative multiple factors. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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