A Fluorescent Dye Method Suitable for Visualization of One or More Rat Whiskers

Autor: Jacopo Rigosa, Alessandro Lucantonio, Giovanni Noselli, Arash Fassihi, Erik Zorzin, Fabrizio Manzino, Francesca Pulecchi, Mathew Diamond
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Bio-Protocol, Vol 8, Iss 5 (2018)
Druh dokumentu: article
ISSN: 2331-8325
DOI: 10.21769/BioProtoc.2749
Popis: Visualization and tracking of the facial whiskers is critical to many studies of rodent behavior. High-speed videography is the most robust methodology for characterizing whisker kinematics, but whisker visualization is challenging due to the low contrast of the whisker against its background. Recently, we showed that fluorescent dye(s) can be applied to enhance visualization and tracking of whisker(s) (Rigosa et al., 2017), and this protocol provides additional details on the technique.
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