Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs

Autor: Kędrak-Jabłońska Agnieszka, Budniak Sylwia, Krupa Marek, Szczawińska Anna, Reksa Monika, Szulowski Krzysztof, Iwaniak Wojciech
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Journal of Veterinary Research, Vol 61, Iss 4, Pp 427-432 (2017)
Druh dokumentu: article
ISSN: 2450-8608
DOI: 10.1515/jvetres-2017-0069
Popis: Introduction: The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes in biological samples of the liver, brain, and blood.
Databáze: Directory of Open Access Journals