Generation of muscle progenitors from human-induced pluripotent stem cells

Autor:	Ibrahim Elmadbouh
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	Givinostat Pharmacological preconditioning Differentiation of hiPSCs Cell signaling Medicine (General) R5-920 Genetics QH426-470
Zdroj:	Egyptian Journal of Medical Human Genetics, Vol 23, Iss 1, Pp 1-14 (2022)
Druh dokumentu:	article
ISSN:	2090-2441
DOI:	10.1186/s43042-022-00319-4
Popis:	Abstract Background Small molecules have a role in the differentiation of human-induced pluripotent stem cells (hiPSCs) into different cell linages. The aim of this study was to evaluate the differentiation of hiPSCs into cardiac or skeletal myogenic progenitors with a single small molecule. Methods hiPSCs were treated with three different small molecules such as Isoxazole-9, Danazol and Givinostat in serum-free medium for 7 days. Cell viability, qRT-PCR, western blots, and immunostaining were assessed after treatment of hiPSCs with small molecules. Results Higher hiPSC viability was observed in hiPSCs treated with Isoxazole-9 (25 µM), Danazol (25 µM) and Givinostat (150 nM) versus control (P
Databáze:	Directory of Open Access Journals
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