Rapid, reliable and inexpensive quality assessment of biotinylated cRNA
| Autor: | T. Zander, J.A. Yunes, A.A. Cardoso, L.M. Nadler |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 39, Iss 5, Pp 589-593 (2006) |
| Druh dokumentu: | article |
| ISSN: | 0100-879X 1414-431X |
| DOI: | 10.1590/S0100-879X2006000500004 |
| Popis: | The interpretation of oligonucleotide array experiments depends on the quality of the target cRNA used. cRNA target quality is assessed by quantitative analysis of the representation of 5' and 3' sequences of control genes using commercially available Test arrays. The Test array provides an economically priced means of determining the quality of labeled target prior to analysis on whole genome expression arrays. This manuscript validates the use of a duplex RT-PCR assay as a faster (6 h) and less expensive (6 were chosen and classified as degraded cRNAs, and 31 samples with a ß-actin 3'/5' ratio |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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