Comparison of target enrichment strategies for ancient pathogen DNA
| Autor: | Anja Furtwängler, Judith Neukamm, Lisa Böhme, Ella Reiter, Melanie Vollstedt, Natasha Arora, Pushpendra Singh, Stewart T Cole, Sascha Knauf, Sébastien Calvignac-Spencer, Ben Krause-Kyora, Johannes Krause, Verena J Schuenemann, Alexander Herbig |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 69, Iss 6, Pp 455-459 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/btn-2020-0100 |
| Popis: | In ancient DNA research, the degraded nature of the samples generally results in poor yields of highly fragmented DNA; targeted DNA enrichment is thus required to maximize research outcomes. The three commonly used methods – array-based hybridization capture and in-solution capture using either RNA or DNA baits – have different characteristics that may influence the capture efficiency, specificity and reproducibility. Here we compare their performance in enriching pathogen DNA of Mycobacterium leprae and Treponema pallidum from 11 ancient and 19 modern samples. We find that in-solution approaches are the most effective method in ancient and modern samples of both pathogens and that RNA baits usually perform better than DNA baits. |
| Databáze: | Directory of Open Access Journals |
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