Surface IgM-Inducing Factor in the Culture Supernatant of Bursal Epithelial Cells Derived from Chick Embryos.
| Autor: | Song Han, Mineyuki Kondo, Asaki Abe, Yoshinobu Kimura, Kazumasa Ohtsuki, Yasuhiro Kondo |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | The Journal of Poultry Science, Vol 40, Iss 2, Pp 130-138 (2003) |
| Druh dokumentu: | article |
| ISSN: | 1346-7395 1349-0486 |
| DOI: | 10.2141/jpsa.40.130 |
| Popis: | Bursal epithelial cells derived from 14-day-old chick embryos were cultured under a serum-free condition and the recovered culture supernatant was analyzed for surface immunoglobulin M (sIgM)-inducing activity on the bursacytes of 12-day-old chick embryos. Surface IgM positive rate of bursacytes incubated with the culture supernatant increased significantly, thus indicating the presence of sIgM-inducing factor(s) in the culture supernatant. The sIgM-inducing factor(s) in the supernatant was analyzed by gel filtration chromatography and HPLC. Results from these analyses indicated that the factor may be a polypeptide with a molecular weight of about 1300. This substance which is different from already known factors, such as Bursin, may be a newly discovered B cell differentiating factor in the bursa of Fabricius that promotes chicken B cell differentiation. |
| Databáze: | Directory of Open Access Journals |
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