Chemiluminescence based immunoassay for the detection of heroin and its metabolites

Autor: Smritee Singh, Priya Mishra, Ivneet Banga, Avanish S Parmar, Prem Prakash Tripathi, Sonu Gandhi
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: BioImpacts, Vol 8, Iss 1, Pp 53-58 (2018)
Druh dokumentu: article
ISSN: 2228-5660
2228-5652
DOI: 10.15171/bi.2018.07
Popis: Introduction: Continuous use of opiates causes drug-related illnesses, which poses an alarming situation to develop sensitive detection platform. In this study, a highly sensitive and reliable chemiluminescence immunoassay (CI) has been developed for the detection of heroin and its major metabolites in spiked urine samples. Methods: To develop robust immunoassay, monoacetyl morphine-bovine serum albumin (MAM-BSA) conjugate was synthesized and characterized thoroughly by physicochemical techniques. The anti-MAM antibodies were developed, labeled with horseradish peroxidase (HRP) and immunoassay was developed to detect the presence of target drug in spiked urine samples. Results: A competitive CI was developed, where heroin, MAM, morphine, and codeine concentration were ranged from 0-1000 ng/ mL in spiked urine samples and limit of detection were 80, 95, 90, 75 pg/ mL. Conclusion: The developed CI is highly sensitive, specific, point of care, cost-effective and can be used as a routine technique for quantitative analysis for screening of narcotic drugs.
Databáze: Directory of Open Access Journals
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