| Autor: |
Yi Pan, Haihong Zhou, Ablatt Mahsut, Rory J. Rohm, Olga Berejnaia, Olga Price, Ying Chen, Jose Castro-Perez, Michael E. Lassman, David McLaren, James Conway, Kristian K. Jensen, Tiffany Thomas, Gissette Reyes-Soffer, Henry N. Ginsberg, David E. Gutstein, Michele Cleary, Stephen F. Previs, Thomas P. Roddy |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 55, Iss 6, Pp 1179-1187 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1194/jlr.D047829 |
| Popis: |
LC/MS quantification of multiple plasma proteins that differ by several orders of magnitude in concentration from a single sample is challenging. We present a strategy that allows the simultaneous determination of the concentration and turnover kinetics of higher and lower abundant proteins from a single digestion mixture. Our attention was directed at a cluster of proteins that interact to affect the absorption and interorgan lipid trafficking. We demonstrate that apos involved in TG metabolism such as apoC2, C3, E, and A4 (micromolar concentration), and apoB48 and apoA5 (single-digit nanomolar concentration) can be quantified from a single digestion mixture. A high degree of correlation between LC/MS and immunobased measurements for apoC2, C3, E, and B48 was observed. Moreover, apoA5 fractional synthesis rate was measured in humans for the first time. Finally, the method can be directly applied to studies involving nonhuman primates because peptide sequences used in the method are conserved between humans and nonhuman primates. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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