Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction
Autor: | Marize Pereira Miagostovich, Flávia Barreto dos Santos, Eliane Saraiva M de Araújo, Juarez Dias, Hermann G Schatzmayr, Rita Maria Ribeiro Nogueira |
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Jazyk: | angličtina |
Rok vydání: | 1997 |
Předmět: | |
Zdroj: | Memorias do Instituto Oswaldo Cruz, Vol 92, Iss 5, p 595 (1997) |
Druh dokumentu: | article |
ISSN: | 0074-0276 1678-8060 |
DOI: | 10.1590/S0074-02761997000500006 |
Popis: | A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes in endemic areas |
Databáze: | Directory of Open Access Journals |
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