| Autor: |
Sei-ichi Kawahara, Chisato Ishihara, Kiriko Matsumoto, Shogo Senga, Koichiro Kawaguchi, Ayaka Yamamoto, Jutalak Suwannachot, Yasunori Hamauzu, Hidefumi Makabe, Hiroshi Fujii |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Heliyon, Vol 5, Iss 10, Pp e02610- (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2405-8440 |
| DOI: |
10.1016/j.heliyon.2019.e02610 |
| Popis: |
The aim of the present study was to characterize and evaluate the anti-cancer activity of proanthocyanidin-enriched fractions from adzuki beans. For this purpose, we concentrated proanthocyanidins from adzuki beans (Vigna angularis) into five fractions using Amberlite XAD-1180N, Toyopearl HW40F, and Sepacore C-18 reverse-phase flash column chromatography. Proanthocyanidin-enriched fractions were characterized as (epi)catechin hexamer, heptamer, and octamer, epigallocatechin-(epi)catechin pentamer, and epigallocatechin-(epi)catechin hexamer using electrospray ionization time-of-flight mass spectrometry and thiolytic degradation. These fractions showed significant anti-cancer activity against the human PC-3 prostate cancer cell line. They also significantly suppressed the expression of the fatty acid-binding protein 5 gene, which plays critical roles in cell growth and metastasis in prostate cancer. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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