Arginine-specific gingipains (RgpA/RgpB) knockdown modulates neutrophil machinery

Autor: Vanessa Tubero Euzebio Alves, Tomaz Alves, Emanuel Silva Rovai, Hatice Hasturk, Thomas Van Dyke, Marinella Holzhausen, Alpdogan Kantarci
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Oral Microbiology, Vol 16, Iss 1 (2024)
Druh dokumentu: article
ISSN: 20002297
2000-2297
DOI: 10.1080/20002297.2024.2376462
Popis: Background Gingipains are important virulence factors present in Porphyromonas gingivalis. Arginine-specific gingipains (RgpA and RgpB) are critically associated with increased proteolytic activity and immune system dysfunction, including neutrophilic activity. In this study, we assessed the impact of gingipains (RgpA and RgpB) on neutrophil function.Methods Peripheral blood samples were obtained; neutrophils were isolated and incubated with P. gingivalis A7436, W50, and the double RgpA/RgpB double knockout mutant E8 at MOI 20 for 2 hours. Neutrophil viability was assessed by Sytox staining. Phagocytic capacity and apoptosis were measured by flow cytometry. Superoxide release was measured by superoxide dismutase and cytochrome c reduction assay. Gene expression of TLR2, p47-phox, p67-phox, and P2 × 7was measured by qPCR. Inflammatory cytokine and chemokine production was measured by IL-1β, IL-8, RANTES, and TNF-α in cell supernatants.Results Neutrophil TLR2 gene expression was reduced in the absence of RgpA/RgpB (p 0.05).Conclusion These data suggest that arginine-specific gingipains (RgpA/RgpB) can modulate neutrophil responses against P. gingivalis infection.
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