| Autor: |
J.Y. Chen, L.X. Yang, Z.F. Huang |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Brazilian Journal of Medical and Biological Research, Vol 46, Iss 12, Pp 1021-1027 (2013) |
| Druh dokumentu: |
article |
| ISSN: |
1414-431X |
| DOI: |
10.1590/1414-431X20132833 |
| Popis: |
Siva-1 induces apoptosis in multiple pathological processes and plays an important role in the suppression of tumor metastasis, protein degradation, and other functions. Although many studies have demonstrated that Siva-1 functions in the cytoplasm, a few have found that Siva-1 can relocate to the nucleus. In this study, we found that the first 33 amino acid residues of Siva-1 are required for its nuclear localization. Further study demonstrated that the green fluorescent protein can be imported into the nucleus after fusion with these 33 amino acid residues. Other Siva-1 regions and domains showed less effect on Siva-1 nuclear localization. By site-mutagenesis of all of these 33 amino acid residues, we found that mutants of the first 1-18 amino acids affected Siva-1 nuclear compartmentalization but could not complete this localization independently. In summary, we demonstrated that the N-terminal 33 amino acid residues were sufficient for Siva-1 nuclear localization, but the mechanism of this translocation needs additional investigation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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