| Autor: |
Zaid Taha, Mathieu Joseph François Crupi, Nouf Alluqmani, Duncan MacKenzie, Sydney Vallati, Jack Timothy Whelan, Faiha Fareez, Akram Alwithenani, Julia Petryk, Andrew Chen, Marcus Mathew Spinelli, Kristy Ng, Judy Sobh, Christiano Tanese de Souza, Priya Rose Bharadwa, Timothy Kit Hin Lee, Dylan Anthony Thomas, Ben Zhen Huang, Omar Kassas, Joanna Poutou, Victoria Heather Gilchrist, Stephen Boulton, Max Thomson, Ricardo Marius, Mohsen Hooshyar, Scott McComb, Rozanne Arulanandam, Carolina Solange Ilkow, John Cameron Bell, Jean-Simon Diallo |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Nature Communications, Vol 15, Iss 1, Pp 1-20 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2041-1723 |
| DOI: |
10.1038/s41467-024-51498-0 |
| Popis: |
Abstract Targeted antineoplastic immunotherapies have achieved remarkable clinical outcomes. However, resistance to these therapies due to target absence or antigen shedding limits their efficacy and excludes tumours from candidacy. To address this limitation, here we engineer an oncolytic rhabdovirus, vesicular stomatitis virus (VSVΔ51), to express a truncated targeted antigen, which allows for HER2-targeting with trastuzumab. The truncated HER2 (HER2T) lacks signaling capabilities and is efficiently expressed on infected cell surfaces. VSVΔ51-mediated HER2T expression simulates HER2-positive status in tumours, enabling effective treatment with the antibody-drug conjugate trastuzumab emtansine in vitro, ex vivo, and in vivo. Additionally, we combine VSVΔ51-HER2T with an oncolytic vaccinia virus expressing a HER2-targeted T-cell engager. This dual-virus therapeutic strategy demonstrates potent curative efficacy in vivo in female mice using CD3+ infiltrate for anti-tumour immunity. Our findings showcase the ability to tailor the tumour microenvironment using oncolytic viruses, thereby enhancing compatibility with “off-the-shelf” targeted therapies. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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